DOI: 10.1111/gbi.12068
Scopus记录号: 2-s2.0-84890201291
论文题名: Molecular and lipid biomarker analysis of a gypsum-hosted endoevaporitic microbial community
作者: Jahnke L.L. ; Turk-Kubo K.A. ; N. Parenteau M. ; Green S.J. ; Kubo M.D.Y. ; Vogel M. ; Summons R.E. ; Des Marais D.J.
刊名: Geobiology
ISSN: 1472-4677
EISSN: 1472-4669
出版年: 2014
卷: 12, 期: 1 起始页码: 62
结束页码: 82
语种: 英语
Scopus关键词: abundance
; biomarker
; chlorophyll
; fatty acid
; gypsum
; lipid
; membrane
; microbial community
; Precambrian
; RNA
; Baja California Sur
; Guerrero Negro
; Mexico [North America]
; biological marker
; calcium sulfate
; DNA
; lipid
; article
; bacterium
; chemistry
; classification
; DNA sequence
; genetics
; Mexico
; microbiology
; molecular genetics
; nucleotide sequence
; sediment
; Bacteria
; Biological Markers
; Calcium Sulfate
; DNA
; Geologic Sediments
; Lipids
; Mexico
; Molecular Sequence Data
; Sequence Analysis, DNA
Scopus学科分类: Earth and Planetary Sciences: General Earth and Planetary Sciences
; Environmental Science: General Environmental Science
; Agricultural and Biological Sciences: Ecology, Evolution, Behavior and Systematic
英文摘要: Modern evaporitic microbial ecosystems are important analogs for understanding the record of earliest life on Earth. Although mineral-depositing shallow-marine environments were prevalent during the Precambrian, few such environments are now available today for study. We investigated the molecular and lipid biomarker composition of an endoevaporitic gypsarenite microbial mat community in Guerrero Negro, Mexico. The 16S ribosomal RNA gene-based phylogenetic analyses of this mat corroborate prior observations indicating that characteristic layered microbial communities colonize gypsum deposits world-wide despite considerable textural and morphological variability. Membrane fatty acid analysis of the surface tan/orange and lower green mat crust layers indicated cell densities of 1.6 × 109 and 4.2 × 109 cells cm-3, respectively. Several biomarker fatty acids, {increment}7,10-hexadecadienoic, iso-heptadecenoic, 10-methylhexadecanoic, and a {increment}12-methyloctadecenoic, correlated well with distributions of Euhalothece, Stenotrophomonas, Desulfohalobium, and Rhodobacterales, respectively, revealed by the phylogenetic analyses. Chlorophyll (Chl) a and cyanobacterial phylotypes were present at all depths in the mat. Bacteriochlorophyl (Bchl) a and Bchl c were first detected in the oxic-anoxic transition zone and increased with depth. A series of monomethylalkanes (MMA), 8-methylhexadecane, 8-methylheptadecane, and 9-methyloctadecane were present in the surface crust but increased in abundance in the lower anoxic layers. The MMA structures are similar to those identified previously in cultures of the marine Chloroflexus-like organism 'Candidatus Chlorothrix halophila' gen. nov., sp. nov., and may represent the Bchl c community. Novel 3-methylhopanoids were identified in cultures of marine purple non-sulfur bacteria and serve as a probable biomarker for this group in the lower anoxic purple and olive-black layers. Together microbial culture and environmental analyses support novel sources for lipid biomarkers in gypsum crust mats. © 2013 John Wiley & Sons Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85184
Appears in Collections: 影响、适应和脆弱性
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作者单位: Exobiology Branch, NASA, Ames Research Center, Moffett Field, CA, United States; SETI Institute, Mountain View, CA, United States; EAPS Department, Massachusetts Institute of Technology, Cambridge, MA, United States; Department of Ocean Sciences, University of California Santa Cruz, Santa Cruz, CA, United States; Department of Biological Sciences, University of Illinois Chicago, Chicago, IL, United States
Recommended Citation:
Jahnke L.L.,Turk-Kubo K.A.,N. Parenteau M.,et al. Molecular and lipid biomarker analysis of a gypsum-hosted endoevaporitic microbial community[J]. Geobiology,2014-01-01,12(1)