DOI: 10.1016/j.marpolbul.2014.05.025
Scopus记录号: 2-s2.0-84906264430
论文题名: Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus
作者: Kim S.-H. ; Kim S.-J. ; Lee J.-S. ; Lee Y.-M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2014
卷: 85, 期: 2 起始页码: 455
结束页码: 462
语种: 英语
英文关键词: Euplotes crassus
; Glutathione
; Heavy metals
; Marine ciliate
; Reactive oxygen species
Scopus关键词: Heavy metals
; Oxygen
; Peptides
; Polymerase chain reaction
; Environmental contaminant
; Euplotes crassus
; Glutathione peroxidase
; Glutathiones
; Marine ciliates
; Metal-induced oxidative stress
; Reactive oxygen species
; Transcriptional modulation
; Gene expression
; cadmium
; copper
; glutathione
; glutathione peroxidase
; glutathione reductase
; heavy metal
; lead
; reactive oxygen metabolite
; zinc
; antioxidant
; biological marker
; glutathione
; glutathione peroxidase
; glutathione reductase
; heavy metal
; reactive oxygen metabolite
; water pollutant
; biomarker
; eukaryote
; gene expression
; heavy metal
; marine pollution
; pollution effect
; acute toxicity
; article
; cellular immunity
; controlled study
; enzyme activity
; Euplotes
; Euplotes crassus
; gene expression
; genetic transcription
; GPx gene
; GR gene
; heavy metal poisoning
; nonhuman
; nucleotide sequence
; oxidative stress
; protein content
; risk assessment
; transcription regulation
; upregulation
; drug effects
; Euplotes
; genetics
; metabolism
; toxicity
; water pollutant
; Ciliophora
; Eukaryota
; Moneuplotes crassus
; Antioxidants
; Biological Markers
; Cadmium
; Copper
; Euplotes
; Glutathione
; Glutathione Peroxidase
; Glutathione Reductase
; Lead
; Metals, Heavy
; Oxidative Stress
; Reactive Oxygen Species
; Water Pollutants, Chemical
; Zinc
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Euplotes crassus, a single-celled eukaryote, is directly affected by environmental contaminants. Here, exponentially cultured E. crassus were exposed to cadmium, copper, lead, and zinc and then the reactive oxygen species (ROS) and total glutathione (GSH) levels were measured. Subsequently, the transcriptional modulation of glutathione peroxidase (GPx) and glutathione reductase (GR) were estimated by quantitative RT-PCR. After an 8-h exposure, significantly higher increases in the relative ROS and total GSH levels were observed in exposed group, compared to the controls. Real-time PCR data revealed that the expression levels of GPx and GR mRNA were sensitively modulated within 8. h of exposure to all heavy metals. These findings suggest that these genes may be involved in cellular defense mechanisms by modulating their gene expression against heavy metal-induced oxidative stress. Thus, they may be useful as potential molecular biomarkers to assess sediment environments for contaminants. © 2014 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85355
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Department of Life Science, College of Natural Science, Sangmyung University, Seoul 110-743, South Korea; Deep-sea and Seabed Resources Research Division, Korea Institute of Ocean Science and Technology (KIOST), Ansan 426-744, South Korea; Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea
Recommended Citation:
Kim S.-H.,Kim S.-J.,Lee J.-S.,et al. Acute effects of heavy metals on the expression of glutathione-related antioxidant genes in the marine ciliate Euplotes crassus[J]. Marine Pollution Bulletin,2014-01-01,85(2)