DOI: 10.1016/j.marpolbul.2015.08.022
Scopus记录号: 2-s2.0-84940704764
论文题名: Accurate assessment of the impact of salmon farming on benthic sediment enrichment using foraminiferal metabarcoding
作者: Pochon X. ; Wood S.A. ; Keeley N.B. ; Lejzerowicz F. ; Esling P. ; Drew J. ; Pawlowski J.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 100, 期: 1 起始页码: 370
结束页码: 382
语种: 英语
英文关键词: Aquaculture
; Benthic ecology
; Biomonitoring
; Foraminifera
; High-throughput sequencing
; Metabarcoding
Scopus关键词: Aquaculture
; DNA sequences
; Fish
; Nucleic acids
; Throughput
; Benthic ecologies
; Biomonitoring
; Foraminifera
; High-throughput sequencing
; Metabarcoding
; Environmental impact
; assessment method
; benthic foraminifera
; bioindicator
; biomonitoring
; ecological impact
; environmental impact assessment
; salmonid culture
; Article
; benthos
; biological monitoring
; controlled study
; DNA sequence
; environmental impact
; foraminifer
; high throughput sequencing
; indicator organism
; macrobenthos
; metabarcoding
; New Zealand
; nonhuman
; Oncorhynchus tshawytscha
; pisciculture
; RNA sequence
; salmonine
; Scotland
; sediment
; animal
; aquaculture
; biodiversity
; chemistry
; classification
; ecosystem
; environmental monitoring
; foraminifer
; genetics
; multivariate analysis
; procedures
; sediment
; New Zealand
; Foraminifera
; Oncorhynchus tshawytscha
; Animals
; Aquaculture
; Biodiversity
; Ecosystem
; Environmental Monitoring
; Foraminifera
; Geologic Sediments
; High-Throughput Nucleotide Sequencing
; Multivariate Analysis
; New Zealand
; Salmon
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Assessing the environmental impact of salmon farms on benthic systems is traditionally undertaken using biotic indices derived from microscopic analyses of macrobenthic infaunal (MI) communities. In this study, we tested the applicability of using foraminiferal-specific high-throughput sequencing (HTS) metabarcoding for monitoring these habitats. Sediment samples and physico-chemical data were collected along an enrichment gradient radiating out from three Chinook salmon (Oncorhynchus tshawytscha) farms in New Zealand. HTS of environmental DNA and RNA (eDNA/eRNA) resulted in 1,875,300 sequences that clustered into 349 Operational Taxonomic Units. Strong correlations were observed among various biotic indices calculated from MI data and normalized fourth-root transformed HTS data. Correlations were stronger using eRNA compared to eDNA data. Quantile regression spline analyses identified 12 key foraminiferal taxa that have potential to be used as bioindicator species. This study demonstrates the huge potential for using this method for biomonitoring of fish-farming and other marine industrial activities. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/85933
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Environmental Technologies, Coastal and Freshwater Group, Cawthron Institute, Private Bag 2, Nelson, New Zealand; Institute of Marine Science, University of Auckland, Private Bag 349, Warkworth, New Zealand; Environmental Research Institute, University of Waikato, Private Bag 3105, Hamilton, New Zealand; Institute of Marine Research, PO Box 1870, Bergen, Norway; Department of Genetics and Evolution, University of Geneva, Switzerland; IRCAM, UMR 9912, Université Pierre et Marie Curie, Paris, France
Recommended Citation:
Pochon X.,Wood S.A.,Keeley N.B.,et al. Accurate assessment of the impact of salmon farming on benthic sediment enrichment using foraminiferal metabarcoding[J]. Marine Pollution Bulletin,2015-01-01,100(1)