DOI: 10.1016/j.marpolbul.2015.09.037
Scopus记录号: 2-s2.0-84951085430
论文题名: Quantifying indicatively living phytoplankton cells in ballast water samples - recommendations for Port State Control
作者: Gollasch S. ; David M. ; Francé J. ; Mozetič P.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2015
卷: 101, 期: 2 起始页码: 768
结束页码: 775
语种: 英语
英文关键词: Ballast water discharge standards
; Compliance monitoring and enforcement
; Epifluorescence microscopy
; Indicative analysis
; Phytoplankton
; Pulse-amplitude modulated fluorometry
Scopus关键词: Amplitude modulation
; Fluorometers
; Phytoplankton
; Pulse amplitude modulation
; Regulatory compliance
; Ballast water
; Compliance monitoring
; Epifluorescence microscopy
; Indicative analysis
; Pulse-amplitude-modulated fluorometry
; Water
; ballast water
; biomonitoring
; discharge
; identification method
; microbial activity
; microbial community
; phytoplankton
; port operation
; quantitative analysis
; standard (reference)
; Article
; cell count
; cell line
; cell size
; concentration (parameters)
; epifluorescence microscopy
; fluorometry
; nonhuman
; phytoplankton
; phytoplankton cell
; pulse amplitude modulated fluorometry
; water analysis
; water sampling
; devices
; environmental monitoring
; marine biology
; microbiology
; procedures
; ship
; algae
; 3',6'-diacetylfluorescein
; coloring agent
; fluorescein derivative
; Coloring Agents
; Environmental Monitoring
; Fluoresceins
; Fluorometry
; Marine Biology
; Phytoplankton
; Ships
; Water Microbiology
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Different phytoplankton analysis methods (pulse-amplitude modulated fluorometry (PAM) and microscopy) were compared in preparation for compliance monitoring and enforcement with ballast water discharge standards. The key objective was to practically evaluate the performance of different new methods and tools to identify indicatively living phytoplankton cells of the size <. 50. μm in minimum dimension and ≥. 10. μm in minimum dimension as addressed by the Ballast Water Performance Standard (Regulation D-2, International Convention for the Control and Management of Ships' Ballast Water and Sediments, 2004). Four different PAM instruments were selected for the tests based upon knowledge and experience gained in different ballast water sample processing studies. The measurements of the PAM instruments were compared with epifluorescence microscope algae cell counts using fluorescein diacetate as viability stain. It was concluded that PAM fluorometry is a suitable method for indicative phytoplankton analysis of ballast water and the most accurate PAM instruments were identified. © 2015 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/86408
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
There are no files associated with this item.
作者单位: Gollasch Consulting, Grosse Brunnenstrasse 61, Hamburg, Germany; Dr. Matej David Consult d.o.o., Korte 13 e, Izola, Slovenia; National Institute of Biology, Marine Biology Station, Fornace 41, Piran, Slovenia
Recommended Citation:
Gollasch S.,David M.,Francé J.,et al. Quantifying indicatively living phytoplankton cells in ballast water samples - recommendations for Port State Control[J]. Marine Pollution Bulletin,2015-01-01,101(2)