| 项目编号: | 1603943
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| 项目名称: | Collaborative Research: Dynamics of Circular Macromolecules (DNA): From Single Molecules to Highly Entangled States |
| 作者: | Gregory McKenna
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| 承担单位: | Texas Tech University
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| 批准年: | 2016
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| 开始日期: | 2016-08-01
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| 结束日期: | 2019-07-31
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| 资助金额: | 173712
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | bio-molecule
; dynamics
; dna sequencing
; circular dna
; dna synthesis
; dna molecule
; polymer molecule
; single molecule polymer dynamics
; linear entangled dna
; linear molecule
; monodisperse dna sample
; usual linear polymer molecule
; linear dna molecule
; linear dna
; polymer dynamics
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| 英文摘要: | PI: McKenna, Gregory B. / Schroeder, Charles / Anderson, Rae
Proposal Number: 1603943/ 1604038 / 1603925
The goal of this proposal is to explore the behavior of polymer molecules that form large rings, instead of the usual linear polymer molecules. Such polymers, an example of which can be the DNA molecule, behave in a different way than linear molecules when processed or when they flow in a solution, because there are no ends in the chains. Results of this work can lead to improved polymer materials, to understanding in detail the behavior of bio-molecules, and to new technologies for DNA sequencing.
Circular polymers are fascinating materials that have inspired polymer theorists and experimentalists for decades. The dynamics of circular chains differ fundamentally from their linear counterparts due to the absence of chain ends. Despite recent progress, however, the effects of circular topology on polymer dynamics remain a key unresolved problem in the field. In this proposal, the PIs are poised to make major progress in our understanding by preparing circular and linear DNA molecules that are monodisperse and of high topological purity. The assembled team has the expertise to synthesize and characterize circular and linear DNA, and will study the rheological behavior of these materials over a wider range of concentrations and molecular weights than previously achieved. A comprehensive approach is proposed that will include macroscopic and micro-rheology, single molecule polymer dynamics, and DNA synthesis, to provide new information regarding the dynamics of linear and circular DNA. Beyond providing a point of departure for understanding their circular counterparts, the parallel study of linear entangled DNA will provide unprecedented data using perfectly monodisperse DNA samples to directly test predictions from reptation theory, such as the cross-over to reptative behavior at extremely high entanglement densities. In addition to graduate student participation, educational activities are proposed in all three collaborating institutions, ranging from underrepresented minority student involvement at Texas Tech, to high school teacher engagement at Illinois and undergraduate student participation at the U of San Diego, a mainly undergraduate institution. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91554
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Gregory McKenna. Collaborative Research: Dynamics of Circular Macromolecules (DNA): From Single Molecules to Highly Entangled States. 2016-01-01.
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