| 项目编号: | 1542727
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| 项目名称: | RAPID: Response to the MTBE Spill, Port of Houston, Texas, March 2015 |
| 作者: | Kevin Finneran
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| 承担单位: | Clemson University
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| 批准年: | 2014
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| 开始日期: | 2015-06-01
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| 结束日期: | 2016-05-31
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| 资助金额: | USD49991
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | mtbe
; port
; mtbe contamination
; houston
; response
; datum
; mtbe spill
; future engineering response
; long term response
; mtbe remediation
; microbial population
; surface response
; houston stakeholder
; high volume spill response
; mtbe degradation
; oil spill
; mtbe-degrading enrichment culture
; large mtbe spill
; 1542727finneranon march
; native mtbe attenuation rate
; houston shipping lane
; mtbe degrading microbial reaction
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| 英文摘要: | 1542727
Finneran
On March 9 two ships collided in the Port of Houston shipping lane. The Carla Maersk, which was carrying 216,000 barrels of methyl tertiary-butyl ether (MTBE), was ruptured and an estimated 200,000 gallons of MTBE leaked into the Port of Houston. MTBE is extremely water soluble, and visible sheens were absent within a day or two. Therefore, the "surface response" of placing hydrophobic oil skimming booms did not contain the MTBE, and a large volume likely diluted and dispersed within the channel. It is unlikely that standard "oil spill" responses will attenuate MTBE, and this research will provide the Port of Houston stakeholders with data to assist in the short and long term response.
The broad objective of the proposed work is to characterize how the microbial community responds to MTBE contamination in marine sediment, and to identify specific microbial processes that will attenuate MTBE with or without engineering intervention. This is the first time a spill can be tracked from its inception, and the data will assist in all future efforts related to both MTBE remediation and high volume spill response. The specific objectives are to: 1) use metagenomic sequencing and high throughput 16S rRNA gene sequencing to determine both the shifts in microbial populations, as well as microbial activity related to the MTBE spill, and 2) quantify the native MTBE attenuation rates, and identify processes that can increase the rate and extent of biodegradation. These objectives will be met by testing the following hypotheses: 1) The microbial community will shift in response to MTBE and be dominated by operable taxonomic units previously identified in MTBE-degrading enrichment cultures and sediment incubations; and, 2) Sulfate-reducing microorganisms will dominate all MTBE degrading microbial reactions. The research will use high throughput sequencing of microbial DNA extracted from marine sediment in the Port of Houston to characterize the shift amongst microbial populations as they respond to MTBE contamination. In addition, the PI will use standard anoxic batch incubations to determine the rate and extent of MTBE degradation, and the projected pathway of biodegradation by analyzing known intermediates such as tert-butyl alcohol and tert-butyl formate. All anoxic incubation techniques have been previously reported and will be standard anoxic, glass serum bottles sealed with a butyl rubber stopper and sampled with anoxic-gas flushed syringe and needle setups. The proposed activity will be felt at the responder and practitioner levels. These data can and will be used to assist all future engineering responses to large MTBE spills. In addition, the basic scientific data can be used to identify specific microbial populations that respond to and possibly degrade MTBE. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/94555
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Kevin Finneran. RAPID: Response to the MTBE Spill, Port of Houston, Texas, March 2015. 2014-01-01.
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