| 项目编号: | 1450216
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| 项目名称: | BRAIN EAGER: Genetically Encoded Light Sources for Non-Invasive Optogenetics |
| 作者: | Ute Hochgeschwender
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| 承担单位: | Duke University
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2015-02-28
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| 资助金额: | USD300000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | light source
; optogenetic sensor
; optogenetic
; non-invasive light activation
; brain eager
; light output
; lightsensing opsin
; non-invasive optogeneticssignificancethe realization
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| 英文摘要: | PI: Hochgeschwender, Ute H.
Proposal: 1450216
Title: BRAIN EAGER: Genetically Encoded Light Sources for Non-Invasive Optogenetics
Significance
The realization of light-driven genetically targeted neuronal activation and silencing has led to unprecedented possibilities in manipulating neuronal activity in the behaving experimental animal.
However, translation of this approach into the clinical arena for potential therapeutic applications is complicated by the need for implanting optical fibers in the brain as the light source for activating lightsensing opsins. This proposal describes an integrated research, education, and outreach program which focuses on developing a new generation of genetically encoded light sources for non-invasive manipulation of optogenetic sensors. If successful this will be a key threshold advance that will provide the foundation for new technologies enabling minimally invasive and highly efficient diagnostics and therapies. Currently there are no alternative approaches which would achieve, non-invasively, the full range of photonic control of neurons as proposed here.
Technical Description
The investigators will build on the highly innovative concept of combining optogenetics with bioluminescence. To exploit the concept?s potential for non-invasive light activation of optogenetic sensors in clinical settings, they will utilize protein engineering to both improve light output and extend the emission spectrum of the luciferase by optimizing intramolecular bioluminescence resonance energy transfer (BRET) between Gaussia luciferase and various fluorescent proteins. They will test the novel constructs for their efficiency in activating channelrhodopsins and proton and chloride pumps in vitro. The development of these concepts and reagents will have potentially transformative and broad impacts on the implementations of optogenetics in medicine. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95528
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Ute Hochgeschwender. BRAIN EAGER: Genetically Encoded Light Sources for Non-Invasive Optogenetics. 2013-01-01.
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